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Quantification of intensity of histochemical and immunohistochemical responses using standard computer software

12 March 2011
Written by Startseva M.S., Chertok V.M.

  UDK: 611.8.018.82:004 | Pages: 121–123 | Read full textDownload PDF 

Annotation:

The paper describes experience in applying software programs Adobe Photoshop and Mathcad to automatically measure intensity of the responses in cerebral neurons. The authors have used nitroxidergic and serotoninergic neurons of single nucleus detected by histochemical and immunohistochemical methods, respectively, as specimen needed to demonstrate the posibilities of applying the software.

Links to authors:

M.S. Startseva, V.M. Chertok
Vladivostok State Medical University (2 Ostryakova Av. Vladivostok 690950 Russian Federation)

  1. Agroskin L.S., Papajan G.V. Cytometry. Apparatus and methods
    for the analysis of cell absorption. L.: Nauka. 1977. 624 p.
    2. Afanasev A.A., Chertok V.M. Quantitative biomicroscopy micro‑
    vasculature, Pacific Medical Journal. 2004. No. 2. P. 82–86.
    3. Zhuravleva T.B., Prochuhanov R.A. Introduction to quantitative
    histochemistry of enzymes. M.: Mir, 2002. 234 p.
    4. Irjanov Ju.M., Silanteva T.A., Gorbach E.V. Processing and image
    analysis in histological researches by using standard computer
    programs, Morfologicheskie vedomosti. 2004. No. 12. PS. 11–13.
    5. Kocjuba A.E., Chertok V.M., The spatial organization of sero‑
    tonergic and ergic neurons in some nuclei of bulbar department
    of human cardiovascular center, Pacific Medical Journal. 2010.
    No. 4. P. 43–46.
    6. Luppa H. Fundamentals histochemistry. M.: Mir, 1980. 344 p.
    7. Nedzved A.M., Ablamejko C.B. The representation of color
    images for the mathematical morphology, Kompjuternyj analiz
    dannyh i modelirovanie: cb. nauch. statej V mezhdunar. konf.
    / eds. S.A. Ajvazjana i Ju.S. Harina. Minsk: BGU, 1998. Part 4.
    P. 86–95.
    8. Silanteva T.A., Gorbach E.N. A quantitative assessment for the
    intensity histological reactions to digitized images of histological
    preparations with using graded standards, Ukrainskij zhurnal
    telemediciny. 2010. No. 1. P. 68–71.
    9. Starceva M.S., Kocjuba A.E., Koldaev V.M. The usege of systems
    analysis to assess the significance of ergic neurons changes in the
    nucleus of the rats medulla oblongata with experimental hyper‑
    tension, Pacific Medical Journal. 2011. No. 3. P. 61–64.
    10. Chertok V.M., Afanasev A.A., Kocjuba A.E. The usege of au‑
    tomated image analysis system Allegro‑MC for morphometric
    researches, Morfologija. 2003. No. 4. P. 88–92.
    11. Chertok V.M., Kocjuba A.E., Kocjuba E.P. Serotonin and ni‑
    troxidergic neurons in the nucleus of the medulla oblongata,
    Morfologija. 2011. No. 1. P. 32–37.

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