Marine proteobacterium glycopolymers as promising activators of human innate immunity cells

27 May 2011
Written by Smolina T.P., Kuznetsova T.A., Nazarenko E.L., Besednova N.N.

  UDK: 577.114:612.112.91/.94/.95:612.017.11 | Pages: 59–62 | Read full textDownload PDF 


The paper describes effects from lipopolysaccharide (LPS) extracted from marine proteo‑bacterium Pseudoalteromonas nigrifaciens (strain КММ156) and its structural components on the ac‑tivation of neutrophils, monocytes and natural killer cells in human blood. The cell activation was as‑sessed by the changes in expression levels of membranous cluster differentiation (CD) on the natural killer cell surface (CD69 и CD25), membranes of monocytes and neutrophils (CD14 и CD16). The lipopolysaccharide and its and lipid‑free components have activated the innate immunity cells. The lipopolysaccharide caused shedding of membrane molecules СD14 with monocytes and, compared to the control group, considerably increased СD14+СD16+ cell population with a number of effector prop‑erties and a great potential for antigen‑presenting activity. O‑Specific Polysaccharide and core oligosaccharide have increased population of СD14+СD16monocytes to a lesser extent. Enhancing expression of signalling molecules (СD14) and immunoglobulins (СD16) on the neutrophils, the glycopolymers have induced the cells activation. In case of natural killer cells, the lipopolysaccharides have to a greater extent enhanced expression of CD25 (cell‑doubling capacity), and the O‑specific polysaccharide and core oligosaccharide has induced expression of CD69 (marker of cytotoxic functions).

Links to authors:

T.P. Smolina, T.A. Kuznetsova, N.N. Besednova
Research Institute of Epidemiology and Microbiology of the Siberian Brach of RAMS (1 Selskaya St. Vladivostok 690087 Russian Federation)
E.L. Nazarenko
Pacific Institute of Bioorganic Chemistry of FEBRAS (159 100 Year Anniversary of Vladivostok Av. Vladivostok 690022 Russian Federation)

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