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Glycosylation in C-reactive protein in patients with ischemic heart disease

18 March 2011
Written by Chikalovets I.V., Chernikov O.V., Molchanova V.I.

  UDK: 616.12‑005.4‑072:577.112.4 | Pages: 87–90 | Read full textDownload PDF 

Annotation:

The new affine sorbent allowed to develop C‑reactive protein excretion scheme. The pool of serum from patients with ischemic heart disease was used as source to obtain CRP. The carbohydrate‑binding proteins – lectins – allowed to find out that the CRP carbohydrate chains contained the monosaccharide residues of N-acetylgalactosamine, galactose, mannose, N‑acetylglucosamine, and fucose. The lectin binding intensity with immobilised on the CRP plate decreased in the line ‘galactose/N‑acetylgalactosamine‑specific – N‑acetylglucosamine/N‑acetylgalactosamine‑specific – LAA – concanavallin A’. The binding constants with respect to galactose/N‑acetylgalactosamine‑specific and N‑ acetylglucosamine/N‑ acetylgalactosamine‑specific lectins were 1.17 and 0.72 µm–1, respectively.

Links to authors:

I.V. Chikalovets, O.V. Chernikov, V.I. Molchanova
Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of Russian Academy of Sciences (159 100 Year Anniversary of Vladivostok Av. Vladivostok 690022 Russian Federation)

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