Home

Genes expression of Wnt signaling pathway during regenerative process and asexual reproduction in holothurians

Written by Girich A.S., Dolmatov I.Yu

  UDK: 577.218:591.16:593.96 | Pages: 31–33 | Full text PDF | Open PDF 

Annotation:

Background.
Wnt proteins are the key factors in many morphogenetic processes including the regenerative process. Mammals compared with invertebrates are definitely limited in the ability to regenerate the damaged organs although they have the same conservative molecules of Wnt signaling pathways. To activate regenerative process using Wnt proteins it is essential to study the recovery process in model organisms - echinoderms.
Methods. Holothurians were taken from Peter the Great Bay, Sea of Japan as well as from the Gulf of Nha Trang, South China Sea. Evisceration of the holothurians Eupentacta fraudatrix was induced by the
injection of distilled water into the coelomic cavity. On the 3rd, 5th and 7th day after evisceration the aqua pharyngeal complex rudiments and anterior area of recovering intestine were prosected. Holothurian Cladolabes schmeltzii was dissected in the middle part of the body into two fragments. The recovering intestine was prosected on the 14th and 24th day. In addition there were also the organisms engaged in
the process of asexual reproduction (for the gene activity analysis the tissues from the fission spot have been studied). With the sequencing of the transcripts in holothurians the presence of genes WNT1,
WNT2, WNT4, WNT6, WNT7, WNT9, WNT10, WNT16 has been determined during regenerative process and asexual reproduction. 
Results. In E. fraudatrix the genes expression WNT4, WNT6 occurred only at the late phases of regenerative process. In C. schmeltzii WNT2, WNT10, WNT16 activated at the early phases of recovery, WNT1,
WNT2, WNT3, WNT4 and WNT7 – at the late phases. With asexual reproduction of C. schmeltzii the transcripts WNT1, WNT2, WNT4, WNT7, WNT10 have been revealed. The other members of Wnt signaling
pathway have also been found: β-catenin, frizzled, dishevelled. 
Conclusions. The differences in the spectrum of Wnt signaling molecules in various species of holothurians obviously reflect different morphogenesis mechanisms in their anterior and back body structures.
Identified WNT genes sequences have only 45–67 % of the similarity level with homologous genes of the sea urchin Strongylocentrotus purpuratus, which is explained by a large evolutionary gap
between the sea urchins and holothurians. Perhaps in the studied processes WNT genes make a concentration gradient, thereby determining the organs’ rudiment areas or they directly control the regenerative
process, making the cells to migrate and form the organs.

Links to authors:

A.S. Girich1, I.Yu. Dolmatov1, 2
1 Institute of Marine Biology named after A.V. Zhirmunskiy, FEB RAS (17 Palchevskogo St. Vladivostok 690041 Russian Federation),
2 Far Eastern Federal University (8 Sukhanova St. Vladivostok 690091 Russian Federation)

  1. Dolmatov I.Yu., Mashanov V.S. Regeneration in sea cucumbers. Vladivostok: Dalnauka. 2007. 212 p.
  2. Dolmatov I.Yu., Nguyen An Khan, Kamenev Ya.I. Features of asexual reproduction, evisceration and regeneration in sea cu‑cumbers (Holothuroidea) from Nha Trang Bay of South China Sea // Marine Biology. 2012. Vol. 38, No. 3. P. 227–236.
  3. Kamenev Ya.I. Ultrastructure of the internal organs, asexual reproduction and regeneration in the holothurian Cladolabes schmelt‑zii: abstract of dis. by cand. biol. sciences. Vladivostok, 2013. 24 p.
  4. Kulikova K.V., Kibardin A.V., Gnuchev N.V. [et al.]. Signal Wnt pathway and its importance for the development of melanoma // Modern medical technologies. 2012. Vol. 3. P. 107–112.
  5. Fathke C., Wilson L., Shah K. et al. Wnt signaling induces epithelial differentiation during cutaneous wound healing // BMC Cell. Biol. 2006. Vol. 7, No. 4. URL% http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1388211 (application date: 10.12.2013).
  6. Flatt T., Heyland A. Mechanisms of life history evolution. Oxford university press, 2011. 478 p.
  7. Fu J., Jiang M., Mirando A.J., Yu H.M., Hsu W. Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation // Proceedings of the National Academy of Sciences of the USA. Vol. 106, No. 44. P. 18598–18603.
  8. Kawakami Y., Esteban C.R., Raya M. [et al.]. Wnt/betacatenin signaling regulates vertebrate limb regeneration // Genes & Development. 2006. Vol. 20. P. 3232–3237.
  9. Mashanov V.S., Zueva O.R., Garcia-Arraras E.J. Expression of Wnt9, TCTP, and Bmp1/Tll in sea cucumber visceral regeneration // Gene Expression Patterns. 2012. Vol. 12, No. 1–2. P. 24–35.
  10. Millera M.F., Cohenb E.D., Baggsc J.E. [et al.]. Wnt ligands signal in a cooperative manner to promote foregut organogenesis // Proceedings of the National Academy of Sciences of the USA. 2012. Vol. 109, No. 38. P. 15348–15353.
  11. Ortiz-Pineda P.A., Ramírez-Gómez F., Pérez-Ortiz J. [et al.]. Gene expression profiling of intestinal regeneration in the sea cucumber // BMC Genomics. 2009. Vol. 10. 262 p.
  12. Singhl R., MacDonald B.A., Thomas M.L., Lawton P. Patterns of seasonal and tidal feeding activity in the dendrochirote sea cucumber Cucumaria frondosa (Echinodermata: Holothuroidea) in the Bay of Fundy, Canada // Marine ecology progress series. 1999. Vol. 187. P. 133–145.
  13. Sodergreen E., Weinstock G.M., Davidson E.H. [et al.]. The genome of the sea urchin Strongulocentrotus purpuratus // Science. 2006. Vol. 314, No. 5801. P. 941–952.
  14. Takeo M., Chou W.C., Sun Q. [et al.]. Wnt activation in nail epithelium couples nail growth to digit regeneration // Nature. 2013. Vol. 499. P. 228–232 .
  15. Wu B., Crampton S.P., Hughes C.C. Wnt signaling induces matrix metalloproteinase expression and regulates T cell transmigration // Immunity. 2007. Vol. 26. P. 227–239.

PUBLISHER: "MEDITSYNA DV"

Founded in 1997  |  Editions in a year: 4, Articles in one issue: 30 |  ISSN of print version: 1609-1175  |  Ind.: 18410 (Agency "Rospechat’")  |  Edition: 1000 c.