The cytokine profile structure of nasal secretion in children’s allergic rhinitis

Written by Prosekova E.V., Netesova S.Yu., Zabelina N.R., Sabynych V.A., Sotnichenko S.A.

  UDK: 616.211-002-056.43:612.017.1-053.2 | Pages: 38–42 | Full text PDF | Open PDF 


Background. The results of the cytokine profiles study in various biological substrates for children’s allergic diseases presented in solitary domestic and foreign publications being often contradictory that determines importance of this research.
Methods. There was the research of the cytokine profile of nasal secretion in 120 children with allergic rhinitis (AR), which in 40 cases combined with bronchial asthma. The control tests rates - 20 healthy peers. The levels of the Interleukins (IL) 4, 8 and 13, tumor necrosis factor alpha (TNF?) and interferon gamma (IFN?) were determined by enzyme-linked immunosorbent assay.
Results. The predominance of IL-8, increased synthesis of IL-13 and IL-4 in children with AR in the structure of the cytokine profile has been recorded. During activation of allergic immune inflammation the proportion of IL-13, IL-8 and IFN? was substantially similar. During an exacerbation of the disease the proportion of IL-4 in nasal secretion was significantly higher than in the remission period and in mild clinical course of the disease. The content of IL-8 in nasal secretion during AR exacerbation was significantly higher than in the remission period. In children with isolated AR the IL-8 level was 3.2 times lower than in children with complex lesions of the upper and lower respiratory tract but significantly higher than the control tests rates. During the rhinitis exacerbation a statistically significant increase of IL-4 concentration in nasal secretion has been recorded. The INF? content was definitely different in the disease periods, a correlation with the activity and severity of allergic inflammation as well as the presence of allergic pathology have been revealed. The following violation algorithm of the cytokine local regulation: IL-133+, IFN?2–, IL-83+, IL-43+, TNF?3+ has been determined.
Conclusions. The researches have revealed the information value of the cytokine spectrum monitoring of nasal secretion in children with AR to determine the nature, focus, activity and severity of allergic inflammation as well as the program effectiveness evaluation in pharmacotherapy.

Links to authors:

E.V. Prosekova1, S.Yu. Netesova2, N.R. Zabelina1, V.A. Sabynych1, S.A. Sotnichenko3
1 Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690950 Russian Federation)
2 Regional Children’s Clinical Hospital No. 1 (27 Ostryakova Ave. Vladivostok 690002 Russian Federation)
3 Far East District Medical Center of Federal Medicobiologic Agency (161 100-letiya Vladivostoku Ave. Vladivostok 690022 Russian Federation)

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