Nox induced early dismetaboly of the articular cartilage caused by the intra-articular fractures

Written by Cherepovskiy A.V., Shulepin I.V.

  UDK: 616.718.5-001.5:612.128 | Pages: 42–44 | Full text PDF | Open PDF 


Background. Analysis of activity of the NO synthase (NOS) of the articular cartilage of the knee joint as a result of the intra-articular fractures of the lateral condyle of thibia.
Methods. The NADPH-diaphorase analysis of the hematoxylin and eosin and toluidine blue imbued tissue samples out of the core of lateral condyle of the tibia during operations for osteosynthesis on 15 intra-articular fractures patients.
Results. Elicited a statistically significant increment in activity of the NOS of the chondrocytes in the course of the rehabiltation along with a valid decrement in amount of glycosaminoglycans in cartilage matrix. The given changes available subject to the structural intgrity of the cartilage or in the early period of presence of an isolated osteochondral fragment after impression fracture.
Conclusions. The intra-articular fractures cause an early defect of anabolism of the cartilage related with an increment in activity of the NOS. The optimal period of an operation for osteosynthesis may be within 7–10 days after an injure before degradation of the functional potency of a cartilage.

Links to authors:

A.V. Cherepovskiy1, I.V. Shulepin2
1 Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690950 Russian Federation)
2 Vladivostok clinical hospital No. 2 (57 Russkaya St. Vladivostok 690105 Russian Federation)

1. Kornilov N.N., Novoselov K.A., Kornilov N.V. Modern views on the etiopathogenesis, diagnostics principles and conservative therapy of degenerative-dystrophic diseases of the knee // Traumatology and orthopedics of Russia. 2002. No. 2. P. 47–59.
2. Guidance on internal osteosynthesis / Muller M.E., Allgover M., Villenegger H., Schneider R. / translation from German. M.: Ad Marginem, 1996. 750 p.
3. Cherepovskiy A.V., Dubikov A.I., Nikulin S.V. [et al.]. Activity of nitricoxidesynthase as a marker for the early stages of cartilage dismetaboly in the experiment // Bul. exp. biol. med. 2004. Vol.137, No. 5., P. 589–593.
4. Cherepovskiy A.V., Nikulin S.V., Dubikov A.V., Shumatov V.B. Posttraumatic anterior instability of the knee joint: activity of nitricoxidesynthase and early dismetaboly of articular cartilage // Questions of biological, medical and pharmaceutical chemistry. 2004. No. 4. P. 51–54.
5. Amin A.R., Abramson S.B. The role of nitric oxide in articular cartilage breakdown in osteoarthritis // Cur. Op. Rheum. 1998. Vol. 10, No. 3. P. 263–268.
6. Bredt D.S. Endogenous nitric oxide synthesis: biological functions and pathophysiology // Free Radic. Res. 1999. Vol. 31, No. 6. P. 577–596.
7. Fink C., Fermor B., Weinberg J. [et al.]. The effects of dynamic mechanical compression on nitric oxide production in the meniscus // Osteoarthritis Cartilage. 2001. Vol. 9, No. 5. P. 481–487.
8. Hope V.T., Vincent S.R. Histochemical characterization of neuronal NADPH-diaphorase // Histochem. Cytochem. 1989. Vol. 37. P. 653–661.
9. Jouzeau J.Y., Cipoletta C., Presle N. [et al.]. Modulation of IL-effects on cartilage by NO-synthase inhibitors: pharmacological studies in rats // Osteoarthritis Cartilage. 1999. Vol. 7, No. 4. P. 382–385.
10. Kobayashi K., Mishima H., Hashimoto S. [et al.]. Chondrocyte apoptosis and differential expression of nitric oxide in the medial meniscus following partial meniscectomy // J. Orthop. Res. 2001. Vol. 19, No. 5. P. 802–808.
11. Van der Kraan P.M., Vitters E.L., van Beuningen H.M. [et al.]. Role of nitric oxide in the inhibition of BMP-2-mediated stimulation of proteoglycan syntesis in articular cartilage // Osteoarthritis Cartilage. 2000. Vol. 8, No. 2. P. 82–86.
12. Lohmander L.S. Proteoglycans of joint cartilage // Bailliere›s Clin. Rheum. 1988. Vol. 2, No. 1. P. 37–61.
13. Min B.H., Kim H.J., Lim H. [et al.]. Effects of agening and arthritis disease on nitric oxide productions by human articular chondrocytes // Exp. Mol. Med. 2001. Vol. 33, No. 4. P. 299–302.
14. Nathan C., Xie Q.-W. Nitric oxide synthase: roles, tolls, and controls // Cell. 1994. Vol. 78. P. 915–918. 


Founded in 1997  |  Editions in a year: 4, Articles in one issue: 30 |  ISSN of print version: 1609-1175  |  Ind.: 18410 (Agency "Rospechat’")  |  Edition: 1000 c.