Home

The way of a quick identification of bacteria genus Listeria and pathogenic species of Listeria monocytogenes by means of the multiplex polymerase chain reaction

Written by Starodumova S.M., Zaitseva E.A.

  UDK: 579.869.1:57.083.1:577.2.08 | Pages: 95–97 | Full text PDF | Open PDF 

Annotation:

Background. The wide polymorphism of the Listeria microbes and impermanence of their biochemical and biological characteristics inherent to the freshly isolated cultures of Listeria requires the de‑velopment of quite new approaches to their typing.
Methods. Investigated possibility of the use of the multiplex polymerase chain reaction (PCR) with primers limiting the sequences of genes of Phosphoribosyl pyrophosphate synthetase (prs) and Phosphatidylinositol-specific phospholipase (plcA) for a quick identification of bacteria genus Listeria along with a quick differentiation of the pathogenic species of Listeria monocytogenes. Assessment of efficacy of the PCR tested on 117 Listeria cultures isolated from the different foodstaff and organs of the murine rodents
Results. The use of the multiplex PCR enabled to relate 38 of Liste‑
ria cultures to genus L. monocytogenes while the rest to genus Listeria spp. that coincided with the results of microbiological typing and testing by means of LISTER System.
Conclusions. The proposed multiplex PCR system enables to relatively quickly (within 3 hours) to screen for the Listeria-suspected colonies and to simultaneously identify L. monocytogenes in the samples under research and can be used for nmonitoring Listeria infection during microbiological and biological research.

Links to authors:

S.M. Starodumova1, E.A. Zaitseva1, 2 
1 G.P. Somov Institute of Epidemiology and Microbiology of the SB of the Russian Academy of Medical Sciences (1 Selskaya St. Vladivostok 690087 Russian Federation)
2 Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690950 Russian Federation)

  1. Zaitseva E.A., Pukhovskaya N.M., Musatov Yu. S. [et al.]. Molecular and genetic features and epidemiological significance of strains Listeria monocytogenes isolated from pregnant women and abortion material in the Far East region of Russia // Clin. microbial. antimicrob. chemotherapy. 2007. Vol. 9, No. 1. P. 81–89.
  2. Zaitseva E.A. Analysis system of microbiological and molecular genetic markers for detection of highly virulent strains Listeria monocytogenes: dis. doc. med. sciences. M., 2010. 299 p.
  3. Karpova T.I., Ermolaeva S.A., Lopyrev I.V. [et al.]. New methods for identifying Listeria monocytogenes // Clin. microbiol. antimicrob. chemotherapy. 2001. Vol. 3. P. 266–273.
  4. Tartakovskiy I.S., Maleev V.V., Ermolaeva S.A. Listeria: role in human infectious pathology and laboratory diagnostics. M.: Medicine for everyone, 2002. 200 p.
  5. Bubert A., Hein I., Rauch M. [et al.] Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR // Appl. Environ. Microbiol. 1999. Vol. 65, No.10. P. 4688–4692.
  6. Doumith M., Buchrieser C., Glaser P. [et al.] Differentiation of the major Listeria monocytogenes serovars by multiplex PCR // J. Clin. Microbiol. 2004. Vol. 42. P. 3819–3822.
  7. Lehner A., Loncarevic S., Wagner M. [et al.] A rapid differentiation of Listeria monocytogenes by use of PCR–SSCP in the listeriolysin O (hlyA) locus // J. Microbiol. Methods. 1999. Vol. 34, No. 3. P. 165–171.
  8. Liu D. [ed.] Handbook of Listeria monocytogenes // USA: CRC Press, 2008. 552 p.
  9. Marranzano M., Pitrolo S., Vicari O. [et al.] Presence of Listeria spp. in vegetables // Ann. Ig: Med. Rev. e communita. 1996. Vol.8, No. 5. P. 531–535.
  10. Meinersmann R., Phillips R., Wiedmann M. [et al.] Multilocus sequence typing of Listeria monocytogenes by use of hypervariable genes reveals clonal and recombination histories of three lineages // Appl. Environ. Microbiol. 2004. Vol. 70, No. 4. P. 2193–2203.
  11. Nightingale K., Windham K., Wiedmann M. Evolution and molecular phylogeny of Listeria monocytogenes isolated from human and animal listeriosis cases and foods // J. Bacteriol. 2005. Vol. 187, No. 16. P. 5537–5551.
  12. Sallen B., Rajoharison A., Desvarenne S. [et al.] Comparative analysis of 16S and 23S rRNA sequences of Listeria species // Int. J. Syst. Evol. Bacteriol. 1996. Vol. 46. P. 669–674.
  13. Sue D., Fink, D., Wiedmann, M. [et al.] SigmaB-dependent gene induction and expression in Listeria monocytogenes during osmotic and acid stress conditions simulating the intestinal environment // Microbiology. 2004. Vol. 150. P. 3843–3855.
  14. Vazquez-Boland J.A., Kuhn M., Berche P. [et al.] Listeria pathogenesis and molecular virulence determinants // Clin. Microbiol. Rev. 2001. Vol. 14, No. 3. P. 584–640.

PUBLISHER: "MEDITSYNA DV"

Founded in 1997  |  Editions in a year: 4, Articles in one issue: 30 |  ISSN of print version: 1609-1175  |  Ind.: 18410 (Agency "Rospechat’")  |  Edition: 1000 c.