Genetically caused pathogenicity and antibiotic resistance of the Staphylococcus spp. strains

Written by Sharkova V.A., Layman E.F., Mazur M.E.

  UDK: 579.861.2:577.2 | Pages: 46–49 | Full text PDF | Open PDF 


Background. The urgency of a problem of the infections caused by Gram-positive microorganisms all over the world constantly increases. Staphilococcus cause the considerable part of the outpatient and nosocomial bacteriemia, pneumonia, infections of skin, soft tissues, bones and joints.
Methods. 48 strains of Staphylococcus aureus and 51 strains of Staphylococcus epidermidis isolated from operation and postoperative wounds, the upper respiratory ways of medical staff and an environment of surgical units were studied. The method of polymerase chain reaction in a mode of “real time” was used to define the virulence markers with the sets of specific primers to three genes of pathogenicity (fnbрА, fnbрВ and Luc PVS) and to the meticillinresistant gene (mec A).
Results. In dynamics the increase of frequency of detection of pathogenicity genes and geneovariant combinations is noted. At S. aureus spp. pathogenicity has been genetically determined in 81.8 %, at S. epidermidis spp. – in 4 % of cases (isolated from operation wounds). S. epidermidis spp. isolated from surgical medical staff had pathogenicity genes in 14.3 %, and S. aureus spp. – in 85 % of cases. In postoperative wounds genetic determinants were found with less frequency. In microflora of the environment of surgical hospitals pathogenicity genes are allocated only in 11.1 % of S. epidermidis spp. The meticillin-resistance gene is found out at both kinds of Staphilococcus isolated from various biotops.
Conclusion. The analysis of prevalence of genetic determinants confirms the growth of pathogenicity of the clinical isolates of S. epidermidis and S. aureus in the course of the wound infections and also confirms the fact of horizontal transfer of gene Luc PVS. Stability genes to meticillin at S. epidermidis spp. were found more often, especially at strains isolated of biotests that confirms the role of S. epidermidis in transfer of the mobile genetic element mecА of S. aureus.

Links to authors:

V.A. Sharkova1, E.F. Layman2, M.E. Mazur2
1 Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690950 Russian Federation),
2 Center of Hygiene and Epidemiology in Primorsky Krai (36 Utkinskaya St. Vladivostok 692000 Russian Federation)

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