The analysis of indicators of matrix metal proteinases and their inhibitors before and after the dental implantation

Written by Yugai Yu.V., Golitsyna A.A., Tolmachyov V.E., Markelova E.V.

  UDK: 616.314-089.844-06:577.15 | Pages: 65–67 | Full text PDF | Open PDF 


Background. An important aspect of occurrence of early complications of dental implantation and inflammation in the implantation area is integrity of a connecting tissue surrounding it while its injury influences the balance of the tissue metalloproteinase-1 inhibitor (TIMP1) and matrix metalloproteinase (MMPs) 8 and 9.
Methods. 80 patients at the age of 30–60 years old who underwent dental implantation were examined. In 70 cases the postoperative period had no complications (1st group); at 10 cases there were early complications – an edema and hyperemia of the soft tissues (2nd group). In blood and the mixed oral liquid the levels of MMP‑8, MMP-9 and TIMP1 were determined. For the control authors investigated the blood and the oral liquid of 20 practically healthy people.
Results. Statistically significant increase of the systemic level of ММР-8 is revealed, MMP-9 and TIMP1 has been considerably raised at patients of 2nd group. At patients with not complicated postoperative period local and systemic increase of concentration of MMP-9 and local increase of concentration of TIMP1 is found.
Conclusions. Studying of qualitative and quantitative characteristics of ММРs and their inhibitors is a perspective direction of basic researches which will allow developing the new approaches to diagnostics and treatment of complications at dental implantation.

Links to authors:

Yu.V. Yugai, A.A. Golitsyna, V.E. Tolmachyov, E.V. Markelova
Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690950 Russian Federation)

1. Klisho E.V., Kondakova I.V., Choynzonov E.L. [et al.] Prognostic significance of proteases in patients with squamous cell carcinoma of the head and neck // Bulletin SB RAMS. 2005. No. 2 (116). P. 82–91.
2. Nazarov P.G. Acute phase reactants of inflammation. - St. Petersburg: Nauka, 2001, 423 p.
3. Yugay Yu.V., Tolmachev V.E., Markelova E.V. [et al.] Evaluation of cytokine profile in patients before and after dental implant // Pacific Medical Journal. 2013. No. 1. P. 20–22.
4. Brew K., Dinakarpandian D., Nagase H. Tissue inhibitors of metalloproteinases: evolution, structure and function // Biochim. Biophys. Acta. 2000. Vol. 1477 (1–2). P. 267–283.
5. Fini M.E., Cook J.R., Mohan R., Brinckerhoft C.E. Regulation of matrix metallo-proteinase gene expression // Matrix metalloproteinases / eds. Parks W.C., Mecham R.P. San Diego: Academic Press, 1998. P. 299–356.
6. Kiili M., Cox S.W., Chen H.Y. [et al.] Collagenase-2 (MMP-8) and collagenase-3 (MMP-13) in adult periodontitis: molecular forms and levels in gingival crevicular fluid and immunolocalisation in gingival tissue // J. Clin. Periodontol. 2002. Vol. 29. P. 224–232.
7. Mäkelä M., Salo T., Uitto V.J., Larjava H. Matrix metalloproteinases (MMP-2 and TIMP-9) of the oral cavity: cellular origin and relationship to periodontal status // J. Dent. Res. 1994. Vol. 73, No. 8. Р. 1397–1406.
8. Mine Y, Makihira S, Nikawa H. [et al.] Impact of titanium ions on osteoblast-, osteoclast- and gingival epithelial-like cells // Journal of Prosthodontic Research. 2010. Vol. 54. P. 1–6.
9. Nagase H., Woessner J.F. Matrix metalloproteinases // J. Biol. Chem. 1999. Vol. 274, No. 31. P. 21491–21494.
10. Rai B., Kharb S., Jain R., Anand S.C. Biomarkers of periodontitis in oral fluids // J. Oral Science. 2008. Vol. 50, No. 1. P. 53–56.
11. Séguier S., Gogly B., Bodineau A. [et al.] Is collagen breakdown during periodontitis linked to inflammatory cells and expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival tissue // J. Periodontol. 2001. Vol. 72. P. 1398–1406.
12. Shapiro S.D., Senior R.M. Matrix metalloproteinases: matrix degradation and more // American Journal of Respiratory Cell and Molecular Biology. 1999. Vol. 20. P. 1100–10102.
13. Sorsa T., Tjaderhane L., Salo T. Matrix metalloproteinases in oral deseases // Oral. Dis. 2004. Vol. 10, No. 6. P. 311–318.
14. Tuter G., Kurtis B., Serdar M. [et al.] Effects of phase 1 periodontal treatment on gingival crevicular fluid levels of matrix metalloproteinase- 3 and tissue inhibitor of metalloproteinase-1 // J. Clin. Periodontol. 2005. Vol. 32. P. 1011–1015.


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