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CD56 and CD57 expression by distinct populations of human cytotoxic T lymphocytes

Written by Kudryavtsev I.V., Borisov A.G., Volkov A.E., Savchenko A.A., Serebryakova M.K., Polevschikov A.V.

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Annotation:
Background. Cytotoxic T cell subsets with distinct homing potentials, phenotype and effector functions play an important part in many chronic viral infections and autoimmune diseases. Methods. Using 10-color flow cytometry we characterized cytotoxic T cell subsets based on expression of CD45RA, CD62L, CD27, and CD28 and compared the expression of CD56 and CD57 between these subsets. Results. It was shown that CD56 positive cells were predominantly immature T-cells, expressing CD27 and/or CD28. CD57 was found mainly on the cell membrane of most mature populations, lacking CD27 or both co-stimulation molecules. Co-expression of both antigens was determined exclusively on the most mature populations of T cells, which belonged to effector memory (CD45RA−CD62L−) and terminally differentiated effectors (CD45RA+CD62L+) CD3+CD8+ lymphocytes. Conclusions. According to our data in peripheral blood we can identify several populations of cytotoxic T cell with similar properties and phenotype, that due to imperfection of contemporary classifications belong to perfectly different populations

Links to authors:

I.V. Kudryavtsev1, 2, A.G. Borisov3, A.E. Volkov1, A.A. Savchenko3, M.K. Serebryakova2, A.V. Polevschikov1, 2
1 School of Biomedicine of Far Eastern Federal University (8 Sukhanova St. Vladivostok 690950 Russian Federation),
2 Institute of Experimental Medicine (12 Acad. Pavlov St. St. Petersburg 197376 Russian Federation),
3 Scientific Research Institute of Medical Problems of the North (3 Partizana Djelezniaka St. Krasnoyarsk 660022 Russian Federation).


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