The role of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-2 in tissue remodeling of the nasal mucosa with chronic tobacco smoke toxicity in rats

Written by Lepeyko Yu.B., Dyuyzen I.V., Nevzorova V.A., Gilifanov E.A.

  UDK: 591.421:577.15:613.84 DOI: 10.17238/PmJ1609-1175.2016.3.18–21 | Pages: 18–21 | Full text PDF | Open PDF 


Objective. Analysis of the activity of a number of parameters of the structural remodeling of nasal mucosa with chronic tobacco smoke toxicity in rats. Methods. In 25 male Wistar rats have formed a model of chronic tobacco smoking by dispensing tobacco smoke in a specialized chamber for 9 months. 20 intact animals were in the control group. Using immunoperoxidase reaction was detected on paraffin sections of matrix metalloproteinase 9 (MMP 9) and its inhibitor – tissue inhibitors of matrix metalloproteinases 2 (TIMP 2). Results. In the experiment there was a significant increase of MMP 9 and the area occupied by intracellular enzyme. The most evident changes were observed in the lamina propria. Activity Change TIMP 2 showed the opposite trend. Conclusions. Based on the data about the molecular mechanisms regulating the activity of matrix metalloproteinases, it can be assumed that the pharmacological effect on this enzyme system will ensure the prevention and treatment of gross morphological and functional disorders of the nasal mucosa, occurring in smokers.

Links to authors:

Yu.B. Lepeyko1, I.V. Dyuyzen1, 2, V.A. Nevzorova1, E.A. Gilifanov1
1 Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690950 Russian Federation),
2 A.V. Zhirmunsky Institute of Marine Biology of the FEB RAS (17 Palchevskogo St. Vladivosotok 690022 Russian Federation)

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