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Respiratory burst and metabolism of blood neutrophils in patients with different tolerance to acetylsalicylic acid with an acute coronary syndrome

Written by Grinstein I.Yu., Savchenko A.A., Grinstein Yu.I., Gvozdev I.I., Petrova M.M.

  UDK: 616.13-005.6-085.225 | Pages: 61–65 | Full text PDF | Open PDF 

Annotation:

Objective. The research objective is an analysis of the characteristics of a respiratory burst and the activity of NAD(P)-dependent dehydrogenases in blood neutrophils in sensitive and resistant to acetylsalicylic acid (ASA) patients with acute coronary syndrome (ACS).
Methods. The study included 53 patients with ACS. The resistance / sensitivity to ASA was studied in vitro, incubating the platelets to adenosine diphosphate and ASA. Status respiratory burst of neutrophils was studied by chemical luminescence, and the activity of NAD(P)-dependent dehydrogenases – by bioluminescent method.
Results. In patients resistant to ASA the rate of synthesis of primary and secondary reactive oxygen lowered, the index of luminol- dependent activation of neutrophils decreased, of substrate stimulation of glycolysis and glucose oxidation by the pentose phosphate pathway increased.
Conclusions. In patients with ACS resistant to ASA is marked a decrease in functional activity and disturbances in the metabolism of neutrophils. The possible cause of the condition described above is energy losses of a cell, associated with the increase of the intensity and substrate stimulation of glycolysis and glucose oxidation by the pentose phosphate pathway.

Links to authors:

I.Yu. Grinstein1, A.A. Savchenko2, Yu.I. Grinstein1, I.I. Gvozdev2, M.M. Petrova1
1 Krasnoyarsk State Medical University named after Prof. V.F.Voino-Yasenetsky (1a Partizana Zheleznyaka St. Krasnoyarsk 660022 Russian Federation),
2 Research Institute of Medical Problems of the North (3г Partizana Zheleznyaka St. Krasnoyarsk 660022 Russian Federation)


1. Biochemistry / edited by E.S. Severina. М.: GEOTAR-Media, 2004. 784 p.
2. Grinstein I.Yu., Savchenko A.A., Grinstein Yu.I. [et al.]. Hemostatic and neutrophil functional activity in patients with different sensitivity to acetylsalicylic acid in acute coronary syndrome // Cardiovascular Therapy and Prevention. 2015. Vol. 14, No. 5. P. 29–34.
3. Grinstein Yu.I., Filonenko I.V., Savchenko A.A. [et al.]. A method for diagnosing the resistance to the acetylsalicylic acid: patent No. 2413953 RF. MPK G01N 33/86 (2006.01). Published.
03.10.2009. Bull. No. 7. 8 p.
4. Savchenko A.A., Zdzitovetskiy D.E., Borisov A.G. Immune disorders with the extensive purulent peritonitis. Novosibirsk: Nauka, 2013. 142 p.
5. Savchenko A.A. Determination of NAD(P)-dependent dehydrogenases activity in neutrophil granulocytes with the bioluminescent method // Bulletin of Experimental Biology and Medicine. 2015. Vol. 159, No. 5. P. 656–660.
6. Shkapova E.A., Kurtasova L.M., Savchenko A.A. Indicators of lucigenin- and luminol-dependent chemical luminescence of the blood neutrophils in patients with renal cancer // Bulletin of Experimental Biology and Medicine. 2010. Vol. 149, No. 2. P. 201–203.
7. Borekci A., Gur M., Turkoglu C. Oxidative stress and spontaneous reperfusion of infarct-related artery in patients with ST-segment elevation myocardial infarction // Clin. Appl. Thromb. Hemost. 2014; pii: 1076029614546329.
8. Domingo-Gonzalez R., Martínez-Colón G.J., Smith A.J. [et al.]. Inhibition of neutrophil extracellular trap formation after stem cell transplant by prostaglandin E2 // Am. J. Respir. Crit. Care Med. 2016. Vol. 193, No. 2. P. 186–197.
9. Engler R. Free radical and granulocyte-mediated injury during myocardial ischemia and reperfusion // Am. J. Cardiol. 1989. Vol. 63. P. 19–23.
10. Falk E. Pathogenesis of atherosclerosis // J. Am. Coll. Cardiol. 2006. Vol. 47. P. 7–12.
11. Hamm C.W., Bassand J.-P., Agewall S. [et al.] ESC Guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation: The Task Force for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation of the ESC // European Heart Journal. 2011. Vol. 32, No. 23. P. 2999–3054.
12. Han C.Y., Umemoto T., Omer M. [et al.]. NADPH oxidase-derived reactive oxygen species increases expression of monocyte chemotactic factor genes in cultured adipocytes // J. Biol. Chem. 2012. Vol. 287, No. 13. P. 10379–10393.
13. Kobayashi Y. Neutrophil biology: an update // EXCLI J. 2015. Vol. 14. P. 220–227.
14. Martin M.J. Hypertonic saline inhibits arachidonic acid priming of the human neutrophil oxidase // J. Surg. Res. 2013. Vol. 179, No. 1. P. 39–40.
15. Rosa A.P., Jacques C.E., de Souza L.O. [et al.]. Neonatal hyperglycemia induces oxidative stress in the rat brain: the role of pentose phosphate pathway enzymes and NADPH oxidase // Mol. Cell. Biochem. 2015. Vol. 403, No. 1–2. P. 159–167.

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