Elaboration of the method of cultivation of the limbic epithelial stem cells of the cornea

Written by Dubovikov A.S., Konkieva A.V., Kulikov A.N., Churashov S.V., Chernysh V.F., Blinova M.I., Aleksandrova O.I.

  UDK: 611.841.2:576.362/.5.085.23 | Pages: 73–74 | Full text PDF | Open PDF 


To analyze the possibility of culturing limbal epithelial stem cells, an experiment was performed on rabbits of the Chinchilla breed. Cultivation of cells obtained from fragments of limb tissue was performed on αMEM nutrient environment. On the 8th day, the growth of colonies of epithelial-like and fibroblastlike cells was noted. After treatment with antibodies against cytokeratin 19, the presence of limbal epithelial stem cells in the grown population was confirmed.

Links to authors:

A.S. Dubovikov1, A.V., Konkieva1, A.N. Kulikov1, S.V. Churashov1, V.F. Chernysh1, M.I. Blinova2, O.I. Aleksandrova2
1 Military Medical Academy named after S.M. Kirov (6 Akademika Lebedeva St. Saint-Petersburg 194044 Russian Federation),
2 Institute of Cytology of the Russian Academy of Science (4 Tikhoretskiy Ave. Saint-Petersburg 194064 Russian Federation)

1. Bezdetko P.A., Ilina E.N., Naumova O.V. [et al.]. Features of the formation of corneal epithelium after autograft transplantation with limbal insufficiency in the experiment // Journal of Ophthalmology. 2010. No. 1. P. 64–68.
2. Chernysh V.F., Boyko E.V. Eye burns. The problem state and new approaches. Saint Petersburg: Military Medical Academy named after S.M. Kirov Press, 2008. 136 p.
3. Du Y., Carlson E.C., Funderburgh M.L. [et al.]. Stem cell therapy restores transparency to defective murine corneas // Stem Cells. 2009. Vol. 27, No. 7. Р. 1635–1642.
4. Ocular surface disease: Medical and surgical management / M.J. Mannis, E.J. Holland (eds). New York: Springer-Verlag, 2013. 283 p.
5. Pellegrini G., Traverso C.E., Franzi A.T. [et al.]. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium // Lancet. 1997. Vol. 349. Р. 990–993.
6. Sefat F., McKean R., Deshpande P. [et al.]. Production, sterilisation and storage of biodegradable electrospun PLGA membranes for delivery of limbal stem cells to the cornea // 3rd International Conference on Tissue Engineering, ICTE2013. Procedia Engineering, 2013. P. 101–116.


Founded in 1997  |  Editions in a year: 4, Articles in one issue: 30 |  ISSN of print version: 1609-1175  |  Ind.: 18410 (Agency "Rospechat’")  |  Edition: 1000 c.