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Molecular epidemiological evaluation of the impact of globalization processes on the formation of populations of Salmonella Typhimurium in Primorie and other regions of Siberia and the Far East

Written by Kuznetsova A.A., Shubin F.N., Rakov A.V., Yakovlev A.A.

  UDK: 616.98:579.842.14–036.22(571.6+571.1/5) | Pages: 47–53 | Full text PDF | Open PDF 

Annotation:

Objective. Because of product imports from different countries there is a pronounced migration of serotypes of salmonella into Russia, and there is a risk of import of more highly-virulent and antibiotic-resistant microorganisms with contaminated products.
Methods. We have studied 457 strains of Salmonella Typhimurium, isolated from sick people, and 29 strains separated from food products samples in Siberia and Russian Far East. We have analyzed the plasmid features of 486 strains obtained from different ecological origins; sensitivity to antibiotics was studied by discdiffusion method on Müller–Hinton agar in 102 of them.
Results. It was established that population of S. Typhimurium, developed in different regions of Siberia and Russian Far East, is heterogeneous both in plasmid features and in sensitivity to antibiotics. The population is formed because of contaminated products delivery from different regions of Russia and abroad. Generally, imported strains of S. Typhimurium caused focal morbidity; meanwhile strains separated from local meat products caused sporadic morbidity. Strains of S. Typhimurium separated from sick people and livestock products in the region had a similar plasmid type and sensitivity to antibiotics; this is the evidence of their epidemiological relation.
Conclusions. To optimize the system of epidemiological surveillance of salmonellosis caused by S. Typhimurium, it is advisable to examine imported livestock products for the presence of salmonella and sensitivity of separated strains to antibiotics.

Links to authors:

A.A. Kuznetsova1, F.N. Shubin1, A.V. Rakov1, A.A. Yakovlev1, 2
1 Research Institute of Epidemiology and Microbiology named after G.P. Somov (1 Selskaya St. Vladivostok 690087 Russian Federation),
2 Pacific State Medical University (2 Ostryakova Ave. Vladivostok 690002 Russian Federation)


1. Briko N.I., Pokrovsky V.I. Globalization and epidemic process // Epidemiology and Infectious Diseases. Current items. 2010. No. 4. P. 4–10.
2. Ivanov A.S. The modern ideas of an antibiotic resistance and antibacterial therapy of salmonellosis // Clinical Microbiology and Antibacterial Chemotherapy. 2009. Vol. 11, No. 4. P. 305–326.
3. Rakov A.V., Shubin F.N., Kuznetsova N.A. Delivery of products contaminated by Salmonella, in Primorye Region, and their realization in population morbidity // Health. Medical ecology. Science. 2015. Vol. 63, No. 5. P. 26–30.
4. Savilov E.D., Mamontova L.D., Astafyev V. A., Zhdanova S.N. Application of statistical methods in the epidemiological analysis. M.: MEDpress-inform, 2004. 112 p.
5. Syomina N.A., Sidorenko S.V., Rezvan S.P. [et al.]. Determination of sensitivity of microorganisms to antibacterial drugs // Clinical Microbiology and Antibacterial Chemotherapy. 2004. Vol. 6, No. 4. P. 306–359.
6. Chekunina S.N. Epidemiological assessment of the factors determining epidemic process of hepatitis A and shigellosis (on model of Primorsky Krai): autoref. dis. ... candidate of medical sciences. Omsk, 2017. 20 p.
7. Shubin F.N, Maslov D.V., Belogolovkina N.A. [et al.]. Theoretical and applied aspects of microbiological monitoring of salmonellosis // Pacific Medical Journal. 2001. No. 2. P. 26–29.
8. Shubin F.N., Rakov A.V., Kuznetsova N.A. [et al.]. Structure of population of Salmonella enteritidis in Primorsky Krai according to the plasmid analysis // Zhurn. microbiol. 2006. No. 3. P. 28–33.
9. Baggesen D.L., Sandvang D., Aarestrup F.M. Characterization of Salmonella enterica serovar Typhimurium DT104 isolated from Denmark and comparison with isolates from Europe and the United States // Journal of Clinical Microbiology. 2000. Vol. 38. No. 4. P. 1581–1586.
10. Kado C.I., Liu S.T. Rapid procedure for detection and isolation of large and small plasmids // J. Bacteriol. 1981. Vol. 145, No. 3. P. 1365–1373.
11. Malorny B., Schroeter A., Bunge C. [et al.]. Evaluation of molecular typing methods for Salmonella enterica serovar Typhimurium DT104 isolated in Germany from healthy pigs // Vet. Res. 2001. Vol. 32. Р. 119–129.
12. Meakins S., Fisher I.S., Berghold C. [et al.]. Antimicrobial drug resistance in human nontyphoidal Salmonella isolates in Europe 2000–2004: A report from the Enter-net International Surveillance Network // Microbial Drug Resistance. 2008. Vol. 14, No. 1. P. 31–35.
13. National Antimicrobial Resistance Monitoring System (NARMS): Enteric Bacteria 2008. Annual Report. URL: http://www.cdc.gov/ narms/annual/2008/NARMS_2008_Annual_Report.pdf (date of access: 25.04.2018).
14. Van Duijkeren E., Wannet W.J.B., Houwers D.J., van Pelt W. Serotype and phage type distribution of Salmonella strains isolated from humans, cattle, pigs, and chickens in the Netherlands from 1984 to 2001 // J. Clinical Microbiology. 2002. Vol. 40. P. 3980–3985.
15. Wasyl D., Sandvang D., Skov M.N., Baggensen D.L. Epidemiological characteristics of Salmonella Typhimurium isolated from animals and feed in Poland // Epidemiol. Infect. 2006. Vol. 134, No. 1. Р. 179–185.

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