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Assessment of interleukin-17 system in children with allergic bronchial asthma

Written by Prosekova E.V., Turyanskaya A.I., Sabynych V.A.

  UDK: 616.248–056.43:612.017.1 | Pages: 37–40 | Full text PDF | Open PDF 

Annotation:

Objective. The study objective is to assess Тh17 subpopulation of T-lymphocytes and to analyze the features of serum interleukins 17А and 17F (IL-17А, IL-17F) in children with allergic bronchial asthma.
Methods. The study includes 60 children with allergic bronchial asthma and 30 healthy children. Withdrawal criteria from the study was virus-induced phenotype, severe course of asthma, administration of immunocorrecting drugs. The material for the study is venous blood. Cells were assessed with on a flow cytofluorimeter MACSQuant (Miltenyi Biotec GmbH, Germany). Immunoenzyme method was used to determine the content of IL-17A, IL-17F with eBiociens reagents (Bender Medsystems GmbH, Austria), IgE with reagents from the company Alkor Bio (St. Petersburg).
Results. In the serum of children with asthma, a high level of IL- 17A was detected, a moderate direct correlation of its concentration with the number of neutrophils in the peripheral blood and eosinophils in the nasal secretion was determined.
Conclusions. Variability of the functional and quantitative characteristics of the interleukin-17 system was determined depending on the prevalence of allergic inflammation, the significance of imbalance in the interleukin-17 system, and the influence of Th17 lymphocytes on various aspects of inflammation and hyperreactivity of the bronchi associated with T-helpers, types 1 and 2.

Links to authors:

E.V. Prosekova, A.I. Turyanskaya, V.A. Sabynych
Pacific State Medical University (2 Ostryakova ave., Vladivostok, 690002, Russian Federation)


1. Borisova T.V., Karaulov A.V., Sokurenko S.I. Cytokines: participation in pathogenesis and perspectives of therapeutic use in bronchial asthma // Clinical Practice. 2010. Vol. 2, No. 2. P. 81–87.
2. Borisova T.V., Sokurenko S.I., Karaulov A.V. Charactristics of cytokines, phenotipic features and neutrophilsphagocytosis activity in asthmatic patients during exacerbation // Clinical Practice. 2013. Vol. 15, No. 3. P. 11–19.
3. Kaidashev I.P. T-cell regulation in atopic diseases // Clinical Immunology. Allergology. Infectology. 2011. No. 9–10. P. 18–21.
4. Krasnitskaya A.S., Polytika A.N. Specific characteristics of cytokine status in patients with chronic tonsillitis of different aetiology // Pacific Medical Journal. 2013. No. 1. P. 62–64.
5. Bronchial asthma in children. The strategy of treatment and prevention: The national program / Ed. N.A. Geppe, N.G. Kolosova, E.G. Kondyurina [et al.]. Moscow, 2017. 160 p.
6. Simbirtsev A.S. Cytokines in the pathogenesis and treatment of human diseases. Saint Petersburg: Foliant, 2018. 512 p.
7. Ciprandi G., De Amici M., Murdaca G. [et al.]. Serum interleukin- 17 levels are related to clinical severity in allergic rhinitis // Allergy. 2009. Vol. 9, No. 64. P. 1375–1378.
8. Doe C., Bafahel M., Siddiqui S. [et al.]. Expression of the T helper 17-associated cytokines IL-17A and IL-17F in asthma and COPD // Chest. 2010. Vol. 5, No. 138. P. 1140–1147.
9. Huang H., Kim H.J., Chang E.J. [et al.]. IL-17 stimulates the proliferation and differentiation of human mesenchymal stem cells: implications for bone remodeling // Cell Death Differ. 2009. Vol. 16. P. 1332–1343.
10. Koga C., Kabashima K., Shiraishi N. [et al.]. Possible pathogenic role of Th17 cells for atopic dermatitis // J. Invest. Dermatol. 2008. Vol. 128. Р. 2625–2630.
11. Louten J., Boniface K., de Waal Malefyt R. Development and function of TH17 cells in health and disease // J. Allergy Clin. Immunol. 2009. Vol. 123, No. 5. P. 1004–1011.
12. Lyoda M., Shibata T., Kawaguchi M. [et al.]. IL-17A and IL-17F stimulate chemokines via MAPK pathways (ERK1/2 and p38 but not JNK) in mouse cultured mesangial cells: synergy with TNF-α and IL-1β // Am. J. Physiol. Renal. Physiol. 2010. No. 3. P. 779–787.
13. Nakae S., Iwakura Y., Sut H. [et al.]. Phenotypic differences between Th1 and Th2 cells and negative regulation by Th17 // J. Leukoc. Biol. 2007. Vol. 81. P. 1258–1268.
14. Otsuka A., Kabashima K. Mast cells and basophils in cutaneous immune responses // Allergy. 2015. Vol. 70. P. 131–140.
15. Seoung J.P., Lee Y.C. Interleukin-17 regulation: an attractive therapeutic approach for asthma // Respiratory Research. 2010. Vol. 11. P. 78.

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