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Fucoidan from the tang Fucus evanescens: immunophenotypic and morphological changes of dendritic cells – effectors of innate immunity

Written by Makarenkova I.D., Ermakova S.P., Akhmatova N.K., Imbs T.I., Semenova I.B., Khotimchenko M.Yu., Besednova N.N., Makarenkov M.A., Zvyagintseva T.N.

  UDK: 615.37: 577.114:582.272:611 | Pages: 75–79 | Full text PDF | Open PDF 

Annotation:

Objective. The study of the mechanism of action of sulfated polysaccharide from the tang Fucus evanescens on the morphological and immunophenotypic characteristics of dendritic cells (DC) – the key effectors of innate immunity – will enable to establish a rational spectrum for its use.
Methods. We investigated a partially acylated sulfated fucoidan from the tang F. evanescens. Immature DCs were obtained from bone marrow cells of BALB/c mice. Fucoidan (100 μg/ml) and commercial TNFα (20 ng/ml) were added as maturation inducers. Immunophenotypic cells were determined by flow cytometry using monoclonal antibodies.
Results. Fucoidan induced the maturation of DC, as evidenced by their immunophenotypic and morphological changes. A pool of mature cells capable of presenting the antigen to naive T-lymphocytes and activating the effector mechanisms of innate immunity was formed.
Conclusions. Thus, the sulfated polysaccharide from F. evanescens, which contributes to the activation of effector cells of innate immunity, can be used as a potential adjuvant to create an antiinfective defense of the body.

Links to authors:

I.D. Makarenkova1, S.P. Ermakova2, N.K. Akhmatova3, T.I. Imbs2, I.B. Semenova3, M.Yu. Khotimchenko4, N.N. Besednova1, M.A. Makarenkov5, T.N. Zvyagintseva2
1 Somov Institute of Epidemiology and Microbiology (1 Selskaya St. Vladivostok 690087 Russian Federation),
2 G.B. Elyakov Pacific Institute of Bioorganic Chemistry (159, 100 let Vladivostoku Ave. Vladivostok 690022 Russian Federation),
3 Mechnikov Scientific Research Institute of Vaccines and Serums (5a Maly Kazenny Lane, Moscow 105064 Russian Federation,)
4 Far Eastern Federal University (8 Sukhanova St. Vladivostok 690091 Russian Federation), 5 Medical Association of the Far Eastern Branch of the Russian Academy of Sciences (95 Kirova St. Vladivostok 690022 Russian Federation)


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